DNA

Part:BBa_K4438101:Experience

Designed by: Neha Adarsh   Group: iGEM22_IISER-Tirupati_India   (2022-10-11)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4438101

T--IISER-Tirupati India--Fluroscence miR-222 (30 min).png

T--IISER-Tirupati India--Fluroscence miR 222 (1 hr).png Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA

Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.


After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.


User Reviews

UNIQa094c40343735387-partinfo-00000000-QINU UNIQa094c40343735387-partinfo-00000001-QINU